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Cu2+胁迫下不同地域棒头草的耐受性研究

摘要:Cu是植物发展必须的微量元素。过量的Cu可以增进植物发展,但跨越植物本身需求,就会对植物发生迫害感化。最近几年来跟着泥土Cu净化的加重,农作物产量与品德逐年降低,严重影响到人们的临盆与生涯。本文选用分歧地区的棒头草为资料,在分歧Cu2+浓度钳制下,经由过程研讨其种子萌生及心理生化特征遭到的影响,旨在为往后人们在挑选Cu净化泥土的修复植物时,可以供给必定的参考价值和迷信根据。研讨得出以下结论1。低Cu2+钳制对棒头草种子萌生及幼苗发展有增进感化,髙 Cu2+钳制下其种子萌生及幼苗发展均遭到显著克制。且在Cu2+钳制下,根对Cu2+敏理性年夜于苗发展,受损害水平也较年夜。两种棒头草比拟,矿区棒头草种子萌生及幼苗发展受铜影响较小。2。盆栽实验,低Cu2+钳制下,两种棒头草的叶绿素、可溶性卵白、可溶性糖含量会增长;膜透性和MDA含质变化不年夜;SOD活性上升,POD及CAT活性无明显变更。跟着Cu2+钳制水平增强,两种棒头草的叶绿素、可溶性卵白、可溶性糖含量逐步下降;膜透性和MDA含质变年夜;SOD活性显著上升,个中在Cu2+浓度为500mg/kg时,明显降低;POD及CAT则保持上升态势。整体来看分歧Cu2+钳制下,棒头草的叶绿素、可溶性卵白、可溶性糖含量及SOD活性为先上升后降低;膜透性、MDA含量、POD及CAT活性在高Cu2+钳制下,显著变年夜,且一向呈上升态势。两种棒头草比拟,矿区棒头草各类心理生化特征受铜影响水平较小。3。经由过程比较得出棒头草对铜的耐受性强弱具有地区差别性,历久生涯在铜矿区的棒头草,受Cu2+迫害小,本身能够构成了特异的抗铜迫害体系。如矿区棒头草的SOD、POD及CAT活性在分歧Cu2+钳制下均高于非矿区,可以高效消除体内的活性自在基;高Cu2+钳制下,可溶性卵白含量高,利于与Cu2+联合,有用下降Cu2+毒性,解释矿区棒头草顺应了铜净化的情况,曾经成为耐铜性较强的植株。

Abstract:Cu is a trace element in plant development must. Excess Cu can enhance plant development, but across the plant itself needs, will happen harm effect on plant. In recent years along with the aggravation of soil Cu purification, yield and character of crop reduced year by year, the serious influence to people's production and life. This paper selects the bifurcation area Polypogon higegaweri for data, differences in Cu2 concentration control, through the influence on its initiation process by seeds and physiological and biochemical characteristics, aimed at people in phytoremediation back pick Cu purifying mud, can provide certain reference value and a fan letter according to the. The research draws the following conclusion 1. Low Cu2 clamp development has stimulative effect on Seed Germination and seedling Polypogon higegaweri, development of high Cu2 damage the seed germination and seedling was significantly restrained. And in the Cu2 control, the root of Cu2 sensitivity in the eve of the seedling development, damage level is eve. Two kinds of Polypogon higegaweri analogy, mine Polypogon higegaweri seed development and seedling by copper less impact initiation. 2. A pot experiment, the low Cu2 clamp, two Polypogon higegaweri chlorophyll, soluble protein, soluble sugar content increases; membrane permeability and MDA content change is not big; the activity of SOD increased, POD and CAT activity had no significant change. Follow the Cu2 clamp level enhancement, two Polypogon higegaweri chlorophyll, soluble protein, soluble sugar content decreased gradually; membrane permeability and MDA content change Nianye; the activity of SOD remarkably increased, one in the Cu2 when the concentration of 500mg/kg, POD and CAT decreased significantly; keep rising. Overall differences Cu2 clamp, Polypogon higegaweri chlorophyll, soluble protein, soluble sugar content and SOD activity first increased and then decreased; membrane permeability, MDA content, POD and CAT activity in the high Cu2 clamp, significant change Nianye, and always is on the rise. Two kinds of Polypogon higegaweri analogy, mine Polypogon higegaweri all kinds of physiological and biochemical characteristics of copper by affecting the level of small. 3. Through the process of comparing the strength of copper tolerance Polypogon higegaweri has area difference, in copper ore district Polypogon higegaweri long-term career by persecution, Cu2 small, can itself constitute a specific anti copper persecution system. Such as mining Polypogon higegaweri SOD, POD and CAT activity were higher than those in non mining area differences in Cu2 control, can efficiently eliminate the activity of free radicals; high Cu2 clamp, the soluble protein content is high, for combined with Cu2, useful to drop Cu2 toxicity, explain mine Polypogon higegaweri conforms to the situation of copper refining, have become resistant to copper stronger plants.

目录:摘要 3-5

ABSTRACT 5-6

1 文献综述 9-21

1.1 重金属污染及研究现状 9-10

1.2 土壤重金属污染的来源与特点 10-11

1.3 重金属对植物的毒害作用 11-16

1.3.1 重金属对植物种子萌发的影响 12

1.3.2 重金属对植物生长的影响 12-13

1.3.3 重金属对植物细胞膜的影响 13-14

1.3.4 重金属对植物光合作用的影响 14

1.3.5 重金属对植物抗氧化酶系统的影响 14-15

1.3.6 重金属对植物细胞超微结构的影响 15-16

1.4 植物对重金属的耐性机理 16-18

1.4.1 植物对重金属的抗性 16-17

1.4.2 植物对重金属的抗性机制 17-18

1.5 重金属污染环境植物修复的主要方法 18-19

1.6 超富集植物的研究现状 19

1.7 本研究的目的和意义 19-21

2 材料与方法 21-28

2.1 棒头草种子萌发生物测定 21-22

2.1.1 实验材料 21

2.1.2 实验试剂及仪器 21

2.1.3 实验设计 21-22

2.2 盆栽实验及生理生化指标的测定 22-27

2.2.1 实验材料 22

2.2.2 实验试剂及仪器 22

2.2.3 实验设计 22-23

2.2.4 实验方法 23-27

2.3 数据统计分析 27-28

3 结果与分析 28-48

3.1 Cu~(2+)胁迫下不同地域棒头草种子萌发的影响 28-33

3.2 Cu~(2+)胁迫下盆栽土培不同地域棒头草生理生化特性的影响 33-48

3.2.1 Cu~(2+)胁迫下不同地域棒头草叶片叶绿素含量的影响 33-35

3.2.2 Cu~(2+)胁迫下不同地域棒头草根系细胞膜透性的影响 35-36

3.2.3 Cu~(2+)胁迫下不同地域棒头草根系和叶片 MDA 的影响 36-38

3.2.4 Cu~(2+)胁迫下不同地域棒头草根系和叶片 SOD 活性的影响 38-40

3.2.5 Cu~(2+)胁迫下不同地域棒头草根系和叶片 POD 活性的影响 40-42

3.2.6 Cu~(2+)胁迫下不同地域棒头草根系和叶片 CAT 活性的影响 42-44

3.2.7 Cu~(2+)胁迫下不同地域棒头草根系和叶片可溶性蛋白含量的影响. 44-46

3.2.8 Cu~(2+)胁迫下不同地域棒头草可溶性糖含量的影响 46-48

4 讨论 48-53

4.1 Cu~(2+)胁迫下不同地域棒头草种子萌发的影响 48

4.2 Cu~(2+)胁迫下不同地域棒头草生长及生理生化特性的影响 48-53

4.2.1 Cu~(2+)胁迫下不同地域棒头草叶片叶绿素含量的影响 48-49

4.2.2 Cu~(2+)胁迫下不同地域棒头草植物细胞膜的影响 49-50

4.2.3 Cu~(2+)胁迫下不同地域棒头草 MDA 的影响 50

4.2.4 Cu~(2+)胁迫下不同地域棒头草抗氧化系统的影响 50-51

4.2.5 Cu~(2+)胁迫下不同地域棒头草可溶性蛋白的影响 51-52

4.2.6 Cu~(2+)胁迫下不同地域棒头草可溶性糖的影响 52-53

5 结论 53-54

参考文献 54-60

致谢 60
编辑 天晴 13

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